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horseradish peroxidase hrp conjugated donkey polyclonal anti sheep igg  (R&D Systems)


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    R&D Systems horseradish peroxidase hrp conjugated donkey polyclonal anti sheep igg
    Horseradish Peroxidase Hrp Conjugated Donkey Polyclonal Anti Sheep Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+sheep+hrp/pmc12970870-42-3-13?v=R%26D+Systems
    Average 95 stars, based on 179 article reviews
    horseradish peroxidase hrp conjugated donkey polyclonal anti sheep igg - by Bioz Stars, 2026-07
    95/100 stars

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    hrp  (Bio-Rad)
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    Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), <t>and</t> <t>HRP-conjugated</t> secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.
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    Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Journal: Frontiers in Veterinary Science

    Article Title: Optimizing ovine brucellosis serodiagnosis: evaluation of recombinant Brucella antigens and multi-antigen combinations for iELISA

    doi: 10.3389/fvets.2026.1775573

    Figure Lengend Snippet: Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Article Snippet: Optimization parameters included: (i) coating concentration of the single antigen or antigen mixture, (ii) serum dilution, and (iii) working dilution of the HRP-conjugated secondary antibody (Rabbit anti-sheep IgG (H/L): HRP; BIO-RAD, 5184–2,504).

    Techniques: Recombinant, Titration, Concentration Assay